Quantitation of venom Antigens from Moroccan vipers in serum by using an Enzyme-Linked Immunosorbent Assay (ELISA) toward improving health vigilance systems

In the present study an ELISA assay was developed and validated for detection determination of concentration snakes venom in biological samples. Individual component each (Cerastes cerastes Macrovipera mauretanica) used as immunogen to raise specific rabbit IgGs order set up a sandwich-type ELISA. Lower limit, linearity, accuracy, precision, reproducibility, reference intervals were determined. The method proved be simple, specific, reproducible, sensitive (detection limit = 0.5 ng/ml) calibration plot based on linear regression analysis (r 0.980) between 0.9 1000 ng/mL concentration, with lower quantification 1.58 ng/mL. intra- interassay coefficient variation ranged from 2,02 4.62% 5.29 7.40%, respectively. specificity tested using vipers, cobra scorpion venom. This detected all viper species without significant cross reactivity other venoms range 0.9–1000 described is sufficiently clinical evaluation. adaptable venoms. potentially useful diagnosis snakebite, monitor antivenom dose, consequently improve national health monitoring systems.